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Published on: February 23, 2024
Micronucleus scoring in urine cytology specimen by DNA-specific stain
Shelly Sharma1, Pranab Dey, Uma Nahar Saikia
1Department of Cytopathology and Gynecological Pathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Objective:
To evaluate the role of micronuclei (MN) scoring in urinary cytology of bladder transitional cell carcinoma (TCC).
Study Design:
Eighty cases of freshly voided urine samples were used for scoring of MN using acridine orange (AO)-stained smears under fluorescence microscope and routine Papanicolaou-stained smears under light microscopy. MN score for cytologic lesions was compared.
Results:
In AO-stained slides, the mean MN scores were 0.171 +/- 0.395 and 1.489 +/- 1.871 in smears with "no malignant cells seen" and TCC cases, respectively. In Papanicolaou-stained slides the mean MN scores were 0.194 +/- 0.419 and 1.84 +/- 1.876 in smears with "no malignant cells seen," and TCC cases, respectively. Independent sample t test showed significant difference of MN scoring in Papanicolaou-stain and AO stain in no malignancy vs. TCC. Pearson's correlation test showed strong positive correlation between mean MN score by AO and Papanicolaou stains and Pearson's correlation coefficient.
Conclusion:
MN score was significantly high in TCC cases, which may be due to chromosomal damage in bladder carcinoma. In difficult cases, MN may help distinguish benign from malignant cells in urine samples. Routine Papanicolaou stain may also be used for MN score because DNA-specific AO-stained smear gives almost similar results.
