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Updated: May 22, 2026

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

Improved ion mobility resolving power with increased buffer gas pressure.

Eric J Davis¹, Kristopher F Grows, William F Siems

¹Washington State University, Department of Chemistry, P.O. Box 644630, Pullman, Washington 99164, United States.

Analytical Chemistry

|May 18, 2012

Summary

This summary is machine-generated.

High-pressure ion mobility spectrometry (HPIMS) achieves higher resolving power in a compact device. This advancement enhances analytical capabilities for complex security and military applications.

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Area of Science:

Analytical Chemistry
Instrumental Analysis
Physical Chemistry

Background:

Ion mobility spectrometry (IMS) is crucial for security and military applications requiring robust analytical instruments.
Existing IMS systems face limitations in resolving power under complex environmental conditions.
Previous research indicated potential for increased IMS resolving power with higher pressure, but performance plateaued at low temperatures.

Purpose of the Study:

To develop a high-pressure IMS (HPIMS) system capable of maintaining high resolving power efficiency across varying pressures.
To achieve unprecedented resolving powers in a small, portable IMS device.
To establish a novel method for calculating ion collision cross-sections using HPIMS data.

Main Methods:

Designed and implemented a novel aperture grid/Faraday plate configuration for the IMS drift tube.
Operated the HPIMS system at elevated pressures (up to 2.5 atm).
Analyzed the relationship between inverse mobility, pressure, and analyte collision cross-section.

Main Results:

The novel HPIMS design maintained 80% resolving power efficiency irrespective of applied pressure.
A 10.7 cm IMS cell achieved a resolving power of 102 at 2.5 atm.
Increased pressure led to enhanced peak-to-peak resolution.
The slope of the inverse mobility/pressure curve correlated with analyte collision cross-section.

Conclusions:

The developed HPIMS system overcomes previous limitations, offering significantly improved resolving power in a compact form factor.
This technology enhances analytical capabilities for demanding security and military applications.
HPIMS provides a new pathway for determining collision cross-sections of analytes.