Related Experiment Video
Updated: May 22, 2026

Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis
Published on: March 20, 2021
Oxidative stress-mediated apoptotic death in human peripheral blood lymphocytes treated with plasma from
R Vasumathy1, B N Pandey, K P Mishra
1Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, India.
Abstract:
The present study is aimed to investigate the effect of plasma from irradiated human blood on the induction of apoptotic death in human peripheral blood lymphocytes. Our results showed that plasma obtained from γ-irradiated human blood showed a significant decrease in viability and an increase in apoptotic death in unirradiated lymphocytes, which is correlated with increase in intracellular reactive oxygen species level. Moreover, a decrease in the activity of superoxide dismutase in lymphocytes further seems to be associated with oxidative stress-mediated apoptotic death. The diffusible factors from irradiated blood were found to be generated from the cellular components of blood. Based on our results, we suggest induction of oxidative stress-mediated apoptotic death in unirradiated lymphocytes by diffusible factors from irradiated blood.
Related Concept Videos
The Intrinsic Apoptotic Pathway
Regulation of Hematopoietic Stem Cells

