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Related Experiment Video

Updated: May 22, 2026

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation
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A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

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Laser speckle flowmetry method for measuring spatial and temporal hemodynamic alterations throughout large

Joshua K Meisner1, Suna Sumer, Kelsey P Murrell

  • 1University of Virginia, Department of Biomedical Engineering, Box 800759, Health System, Charlottesville, Virginia 22903, USA.

Microcirculation (New York, N.Y. : 1994)
|May 18, 2012
PubMed
Summary

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Laser speckle flowmetry (LSF) quantitatively measures microvessel hemodynamics. This technique revealed distinct hemodynamic network responses to occlusion in C57BL/6 and BALB/c mice due to structural differences.

Area of Science:

  • Physiology
  • Biomedical Engineering
  • Microcirculation Research

Background:

  • Assessing microvascular hemodynamics is crucial for understanding tissue perfusion.
  • Laser speckle flowmetry (LSF) offers potential for quantitative microvessel analysis.
  • Existing methods may lack the resolution for individual microvessel network studies.

Purpose of the Study:

  • To validate LSF as a quantitative tool for individual microvessel hemodynamics in complex networks.
  • To investigate if strain-specific microcirculatory structures in C57BL/6 and BALB/c mice influence hemodynamic network responses to occlusion.

Main Methods:

  • LSF velocity measurements were validated in vitro using capillary phantoms and in vivo in mouse dorsal skinfold (DSFWC) and cremaster muscles.
  • Hemodynamic responses to feed arteriole occlusion were assessed using LSF in DSFWCs of C57BL/6 and BALB/c mice.

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Last Updated: May 22, 2026

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation
07:20

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

Published on: August 30, 2017

How to Build a Laser Speckle Contrast Imaging (LSCI) System to Monitor Blood Flow
05:24

How to Build a Laser Speckle Contrast Imaging (LSCI) System to Monitor Blood Flow

Published on: November 11, 2010

Spatial Temporal Analysis of Fieldwise Flow in Microvasculature
09:39

Spatial Temporal Analysis of Fieldwise Flow in Microvasculature

Published on: November 18, 2019

Main Results:

  • LSF demonstrated a reliable linear relationship between normalized speckle intensity and centerline velocity in vitro and in vivo (R(2) ≥ 0.92).
  • This relationship was independent of vessel diameter and hematocrit.
  • Significant differences in microvascular network responses to occlusion were observed between C57BL/6 and BALB/c mice.

Conclusions:

  • LSF is applicable to intravital microscopy for quantitative, network-wide hemodynamic assessment at the individual microvessel level.
  • Structural variations in mouse microcirculation impact hemodynamic responses to vascular occlusion.
  • LSF provides a valuable tool for studying microcirculation in various physiological and pathological conditions.