Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
Transcription Initiation01:47

Transcription Initiation

Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
Transcription Elongation Factors02:35

Transcription Elongation Factors

Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
Transcription Elongation Factors02:35

Transcription Elongation Factors

Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Characterisation of RNA guanine-7 methyltransferase (RNMT) using a small molecule approach.

The Biochemical journal·2026
Same author

Global genetic interaction network of a human cell maps conserved principles and informs functional interpretation of gene co-essentiality profiles.

Cell·2026
Same author

Single-cell exon deletion profiling reveals splicing events that shape gene expression and cell state dynamics.

Nature communications·2026
Same author

Quantitative analysis of genetic interactions in human cells from genome-wide CRISPR-Cas9 screens.

bioRxiv : the preprint server for biology·2026
Same author

CMTR1 directs mitochondrial dynamics during T cell activation through epitranscriptomic regulation of splice isoforms.

Cell reports·2025
Same author

RNMT-dependent RNA cap methylation in health and disease.

The Biochemical journal·2025

Related Experiment Video

Updated: May 22, 2026

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

E2F1-dependent methyl cap formation requires RNA pol II phosphorylation.

Michael Aregger1, Victoria H Cowling

  • 1Division of Cell Signalling and Immunology, College of Life Sciences, University of Dundee, Dundee, UK.

Cell Cycle (Georgetown, Tex.)
|May 18, 2012
PubMed
Summary

E2F1 transcription factor enhances gene expression by promoting methyl cap formation on RNA polymerase II transcripts. This process is crucial for mRNA maturation, expression, and stability, involving increased RNA pol II phosphorylation.

More Related Videos

Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
10:40

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

Published on: December 28, 2016

Related Experiment Videos

Last Updated: May 22, 2026

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
10:40

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

Published on: December 28, 2016

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Cell Biology

Background:

  • Gene expression is fundamental to cell proliferation.
  • The E2F family of transcription factors regulates genes vital for cell cycle progression.

Purpose of the Study:

  • To investigate the role of E2F1 in gene expression beyond its known transcriptional functions.
  • To elucidate the mechanism by which E2F1 influences RNA polymerase II (pol II) transcripts.

Main Methods:

  • Analysis of RNA polymerase II phosphorylation.
  • Assessing methyl cap formation on transcripts.
  • Investigating the recruitment of methyl cap synthetic enzymes.

Main Results:

  • E2F1 was found to promote the formation of the methyl cap on RNA pol II transcripts.
  • E2F1 increases RNA pol II phosphorylation.
  • This phosphorylation is essential for recruiting methyl cap synthetic enzymes.
  • E2F1-dependent methyl cap formation requires upregulation of RNA pol II phosphorylation.

Conclusions:

  • E2F1 utilizes an additional mechanism to promote gene expression through methyl cap formation.
  • The methyl cap is critical for mRNA maturation, expression, and stability.
  • E2F1-mediated RNA pol II phosphorylation is key to this novel gene expression pathway.