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Related Experiment Video

Updated: May 22, 2026

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

Published on: September 14, 2015

Spatiotemporal changes to the subventricular zone stem cell pool through aging.

Brett A Shook¹, David H Manz, John J Peters

¹Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut 06269, USA.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience

|May 18, 2012

Summary

This summary is machine-generated.

Aging significantly reduces neural stem cells (NSCs) in the rodent subventricular zone (SVZ), decreasing olfactory bulb neurogenesis. Despite fewer NSCs, the proportion of actively dividing cells increases, indicating age-related decline occurs after initial NSC proliferation.

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Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis

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Area of Science:

Neuroscience
Stem Cell Biology
Aging Research

Background:

The subventricular zone (SVZ) in adult rodents is a crucial niche for generating new olfactory bulb interneurons.
Previous studies noted a decline in SVZ neurogenesis with age, but specific changes in SVZ neural stem cells (NSCs) remained unclear.

Purpose of the Study:

To conduct a detailed spatiotemporal evaluation of adult SVZ neural stem cells (NSCs) and their role in age-related neurogenesis decline.
To characterize age-associated alterations in NSC number, distribution, and proliferative activity within the SVZ.

Main Methods:

Serial reconstruction of the SVZ in young (3-month-old) and aged (2-year-old) mice to identify and quantify ventricle-contacting NSCs.
Assessment of NSC processes and regenerative units (pinwheels) along the lateral ventricle wall.
Analysis of NSC proliferation rates using markers of mitotic activity.

Main Results:

A significant decline (86%) in total NSCs per mm² and a reduction in NSCs per pinwheel were observed in aged mice compared to young mice.
The number of pinwheel units per mm² decreased by 78% in aged mice, with a spatially uniform decline across the lateral ventricle.
No significant change in the density of actively proliferating NSCs per mm² was found; however, the percentage of actively mitotic NSCs increased with age.

Conclusions:

Age-related olfactory bulb neurogenesis reduction in the SVZ is associated with a substantial loss of total NSCs and pinwheel structures, not a decrease in proliferation rate.
The decline in neurogenesis occurs downstream of NSC proliferation, suggesting altered NSC maintenance or differentiation contributes to reduced neuronal output in aging rodents.