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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: May 22, 2026

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays
10:58

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Published on: December 3, 2010

A simple and fast method for profiling microRNA expression from low-input total RNA by microarray.

Botao Zhao1, Li Jin, Jiali Wei

  • 1School of Life Sciences, Shanghai University, Shanghai 200444, China. zhaobotao@shu.edu.cn

IUBMB Life
|May 18, 2012
PubMed
Summary
This summary is machine-generated.

This study presents a rapid 2-hour method for labeling mature microRNA (miRNA) in total RNA for microarray analysis. The new technique requires only 100 ng of RNA and yields results consistent with real-time PCR.

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Last Updated: May 22, 2026

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays
10:58

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Published on: December 3, 2010

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Mature microRNA (miRNA) expression analysis is crucial for understanding physiological roles and pathological conditions.
  • Microarray technology offers a powerful approach for global miRNA expression profiling.
  • Efficient and sensitive methods are needed for accurate miRNA detection.

Purpose of the Study:

  • To develop a rapid and sensitive method for labeling mature microRNA (miRNA) directly in total RNA for microarray detection.
  • To reduce the time and RNA input required for miRNA expression analysis.
  • To validate the accuracy of the developed method against established techniques.

Main Methods:

  • A novel miRNA labeling protocol involving RNA tailing with poly(A) polymerase.
  • Utilizing reverse transcription and template-switching with moloney murine leukemia virus (MMLV) reverse transcriptase.
  • Direct labeling of small RNA cDNA for subsequent microarray hybridization.

Main Results:

  • The entire prearray sample preparation process was shortened to 2 hours.
  • The method is effective with a minimal starting amount of 100 ng of total RNA.
  • Expression data generated by this method demonstrated high consistency with real-time polymerase chain reaction (PCR) results.

Conclusions:

  • The developed rapid miRNA labeling method significantly streamlines miRNA expression profiling for microarrays.
  • This technique enables sensitive miRNA analysis using limited RNA samples.
  • The method provides a reliable and efficient alternative for global miRNA expression studies.