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Updated: May 22, 2026
Generation of Fluorescent Protein Fusions in Candida Species
Published on: March 4, 2017
[The construction and expression of Saccharomyces albicans pACT1-GFP plasmids]
Jing Sun1, Fen Jia, Ming-Hui Xia
1School of Stomatology of Shandong University, Key Laboratory of Oral Biomedicine of Shandong Province, Jinan 250012, China.
Objective:
To construct strains containing green fluorescent protein (GFP) to study gene regulation in Saccharomyces albicans cells during the infection process.
Methods:
pACT1-GFP was constructed, and Saccharomyces albicans CAI4 was transformed. The expression of GFP in yeast and hyphal compartments was observed with microscopy.
Results:
99% of Saccharomyces albicans cells containing pACT1-GFP fusion displayed significant fluorescence levels both in the yeast and hyphal compartments. The fluorescence intensity in two compartments had no obvious difference.
Conclusion:
pACT1-GFP can be expressed stably in the yeast cells.
