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Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

Published on: July 26, 2019

Development of an influenza virus protein array using Sortagging technology.

Antonia Sinisi¹, Maximilian Wei-Lin Popp, John M Antos

¹Whitehead Institute for Biomedical Research , 9 Cambridge Center, Cambridge, Massachusetts 02142, United States.

Bioconjugate Chemistry

|May 19, 2012

Summary

This summary is machine-generated.

Researchers developed an Influenza virus protein array using Sortagging technology. This novel protein chip enables high-throughput screening for viral antigens and antibodies, advancing infectious disease diagnostics.

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Area of Science:

Biotechnology
Virology
Immunology

Background:

Protein array technology facilitates high-throughput screening of molecular interactions and antigen identification.
Influenza virus infections necessitate advanced diagnostic tools for rapid antigen and antibody detection.

Purpose of the Study:

To develop an Influenza virus protein array utilizing Sortagging technology.
To create a novel protein chip for antigen and antibody screening.

Main Methods:

Utilized sortase-mediated transpeptidation (Sortagging) to immobilize LPETG-tagged Influenza virus proteins.
Proteins were covalently linked via their carboxyl-termini to a Gly3-linker coated amine-glass slide.
Immobilized proteins were detected using specific antibodies.

Main Results:

Successfully generated an Influenza virus protein array (Sortag-protein chip).
Demonstrated the ability to immobilize viral proteins in an oriented fashion without extensive purification.
Validated the chip for antigen and/or antibody screening.

Conclusions:

Sortagging is an effective method for creating oriented protein arrays on solid supports.
The developed Influenza virus protein chip is a promising tool for high-throughput screening in diagnostics.
This technology is scalable for larger viral genomes, potentially enhancing infectious disease research.