Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

High-Performance Liquid Chromatography: Introduction

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:

High-Performance Liquid Chromatography: Types of Detectors

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...

High-Performance Liquid Chromatography: Instrumentation

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.

High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

Gas Chromatography: Types of Detectors-II

Gas Chromatography: Types of Detectors-II

In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Experimental and Multiscale Modeling Insights for Radiation-Driven Neptunium Redox Processes at Elevated Temperatures.

The journal of physical chemistry. A·2026

Same author

Extraction, Characterization, and Stability Studies of Bistriazinyl-Derived Carboxylic Acids.

Industrial & engineering chemistry research·2026

Same author

Going out with a Bang: Absolute Kinetics of Removing Organic Energetic Compounds from Waters Using Hydroxyl Radicals.

Environmental science & technology·2026

Same author

Mechanisms of Hydroxylating Species Production by Dissolved Organic Matter Model Photosensitizers.

Environmental science & technology·2025

Same author

Impacts of molecular architecture on the radiation-induced degradation and reaction kinetics of hydrophobic diglycolamides with the solvated electron and the dodecane radical cation.

Physical chemistry chemical physics : PCCP·2025

Same author

Radical Treatment of Haloacetonitriles in Aqueous Systems: A Kinetic Study.

ACS ES&T water·2025

Search research articles

Related Experiment Video

Updated: May 22, 2026

Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice

Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice

Published on: October 5, 2017

Determination of CMPO using HPLC-UV.

Gracy Elias¹, Gary S Groenewold, Bruce J Mincher

¹Idaho National Laboratory, Chemical and Radiation Measurement Department, P.O. Box 1625, Idaho Falls, ID 83415-2213, USA. gracy.elias@inl.gov

Journal of Chromatography. A

|May 19, 2012

Summary

A new HPLC method accurately measures octyl(phenyl)-N,N-diisobutylcarbamoylmethylphosphine oxide (CMPO) in nuclear fuel solutions. This technique is crucial for monitoring CMPO degradation during radioactive waste processing.

More Related Videos

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection

Published on: December 15, 2015

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

Related Experiment Videos

Last Updated: May 22, 2026

Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice

Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice

Published on: October 5, 2017

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection

Published on: December 15, 2015

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

Area of Science:

Nuclear chemistry
Analytical chemistry
Separation science

Background:

Octyl(phenyl)-N,N-diisobutylcarbamoylmethylphosphine oxide (CMPO) is vital for separating radionuclides from spent nuclear fuel.
Radiolysis degrades CMPO, impacting separation efficiency and necessitating accurate concentration measurement.

Purpose of the Study:

To develop and validate a novel high-performance liquid chromatography (HPLC) method for quantifying CMPO in dodecane.
To assess CMPO concentration and identify degradation products in irradiated samples.

Main Methods:

Development of a novel HPLC method utilizing a low pH buffer, octanol/2-propanol co-solvent system, and UV detection.
Validation of the HPLC method for linearity, sensitivity, accuracy, and precision.
Identification of degradation products using electrospray ionization mass spectrometry (ESI-MS).

Main Results:

The developed HPLC-UV method demonstrated excellent linearity, sensitivity, accuracy, and precision for CMPO determination.
Analysis of irradiated CMPO samples revealed a decrease in CMPO concentration and the formation of degradation products.
ESI-MS identified CMPO-nitric acid complexes, explaining apparent CMPO loss in acidic conditions, irrespective of irradiation.

Conclusions:

The validated HPLC-UV method provides a reliable means to measure CMPO concentration in nuclear fuel processing.
Understanding CMPO degradation pathways and complex formation is essential for optimizing solvent extraction processes.
The method aids in monitoring the integrity of CMPO during radioactive material handling and separation.