Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 22, 2026

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

Improved technique for manually constructing tissue microarrays for large-core arrays.

Shu-Chuan Tsao1, Chun-Chieh Wu, Chien-Hui Wen

  • 1Department of Pathology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.

Applied Immunohistochemistry & Molecular Morphology : AIMM
|May 19, 2012
PubMed
Summary

Preheating tissue cores before embedding in paraffin blocks prevents core loss during sectioning. This simple method improves tissue microarray integrity for high-throughput analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Natural Extracts of <i>Alnus japonica</i> Induce BAK-Dependent Autophagy to Inhibit Liver Cancer Stem Cell Tumorigenesis.

Antioxidants (Basel, Switzerland)·2026
Same author

Blue light exposure exacerbates Western diet-induced hepatic lipid accumulation and injury <i>via</i> suppression of the SIRT1-NR1D1 axis.

The Analyst·2026
Same author

Teaching NeuroImage: Pachymeningitis as the Initial Manifestation of Kikuchi-Fujimoto Disease.

Neurology·2026
Same author

Lower breast cancer risk among women with two Hakka parents in Taiwan: A nationwide population-based study.

Cancer epidemiology·2026
Same author

ISX-TWIST1 reprograms CD47-inflammasome signaling to reshape the immune microenvironment in liver cancer.

Experimental hematology & oncology·2026
Same author

RTA‑408 induces p38‑dependent apoptosis and suppresses cell viability in hepatocellular carcinoma cells.

Hepatic oncology·2026

Area of Science:

  • Histotechnology
  • Biotechnology
  • Pathology

Background:

  • Tissue microarrays (TMAs) facilitate high-throughput analysis by aligning multiple tissue cores in a single block.
  • A significant challenge in TMA preparation is the loss of tissue cores during sectioning, leading to incomplete or folded tissue sections.

Purpose of the Study:

  • To present a simple, economical method to improve tissue core retention during TMA block sectioning.
  • To enhance the integrity of tissue sections for subsequent histological and immunohistochemical analyses.

Main Methods:

  • Tissue cores were preheated on a hot plate to promote fusion with the surrounding paraffin wax.
  • Six TMA blocks were constructed using 528 tissue cores from formalin-fixed, paraffin-embedded human tissue samples.

More Related Videos

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Related Experiment Videos

Last Updated: May 22, 2026

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Main Results:

  • Preheating facilitated good homogenization between tissue cores and surrounding paraffin.
  • Intact tissue sections were consistently obtained, with satisfactory results observed in both hematoxylin-eosin and immunohistochemical staining.
  • The method proved simple, economical, and required no specialized instrumentation.

Conclusions:

  • Preheating tissue cores is an effective strategy to prevent detachment and loss during TMA sectioning.
  • This technique enhances the reliability of TMAs for high-throughput laboratory analysis.
  • The method is readily implementable in standard histology laboratories.