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Related Experiment Video

Updated: May 22, 2026

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
08:28

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Published on: June 30, 2017

Intrinsic differences in adipocyte precursor cells from different white fat depots.

Yazmín Macotela1, Brice Emanuelli, Marcelo A Mori

  • 1Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts,USA.

Diabetes
|May 19, 2012
PubMed
Summary

Adipose precursor cells from different fat depots show distinct behaviors. Targeting these visceral and subcutaneous cells may offer new strategies for treating obesity and metabolic syndrome.

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Area of Science:

  • Metabolic research
  • Adipose tissue biology
  • Obesity research

Background:

  • Obesity and body fat distribution are key risk factors for type 2 diabetes and metabolic syndrome.
  • Intrinsic differences in adipocyte behavior across fat depots contribute to metabolic risk.
  • Adipocyte precursor cells (APCs) are crucial for adipose tissue expansion and function.

Purpose of the Study:

  • To investigate the distinct characteristics of adipocyte precursor cells (APCs) from visceral and subcutaneous white adipose depots.
  • To understand how genetic and environmental factors influence APC behavior and differentiation.
  • To explore potential therapeutic targets for obesity and its metabolic complications.

Main Methods:

  • Isolation and culture of APCs from visceral and subcutaneous fat depots in mice.

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Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
10:28

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

Published on: July 17, 2018

Related Experiment Videos

Last Updated: May 22, 2026

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
08:28

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Published on: June 30, 2017

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
10:28

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

Published on: July 17, 2018

  • Assessment of APC differentiation potential using standard induction cocktails and specific growth factors (BMP-2, BMP-4).
  • Analysis of gene expression patterns in APCs from different depots and under varying dietary conditions.
  • Quantification of APC numbers in different mouse strains and in response to high-fat diet.

    • Main Results:

    • Visceral and subcutaneous APCs exhibit distinct gene expression profiles and differentiation capacities.
    • Subcutaneous APCs differentiate readily, while visceral APCs require BMP-2 or BMP-4 for efficient differentiation.
    • APC numbers increase significantly with high-fat diet exposure, particularly in obesity-prone mice.
    • Intrinsic differences in APCs from visceral and subcutaneous depots are linked to obesity and metabolic syndrome.

    Conclusions:

    • APCs from visceral and subcutaneous depots are dynamic populations with unique properties.
    • Understanding and regulating these APC populations may offer novel therapeutic avenues.
    • Targeting APCs presents a potential strategy for preventing and treating obesity and related metabolic disorders.