Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fingerprinting genomes using PCR with arbitrary primers.

J Welsh1, M McClelland

  • 1California Institute of Biological Research, La Jolla 92037.

Nucleic Acids Research
|December 25, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ecotin protects <i>Salmonella</i> Typhimurium against the microbicidal activity of host proteases.

bioRxiv : the preprint server for biology·2024
Same author

Discretion in decision to receive COVID-19 vaccines and associated socio-economic inequalities in rates of uptake: a whole-of-population data linkage study from Australia.

Public health·2023
Same author

Inequalities in life expectancy in Australia according to education level: a whole-of-population record linkage study.

International journal for equity in health·2021
Same author

YeiE Regulates Motility and Gut Colonization in Salmonella enterica Serotype Typhimurium.

mBio·2021
Same author

Space Medicine: Why Do Recently Published Papers about Telomere Length Alterations Increase our Uncertainty Rather than Reduce it?

Journal of biomedical physics & engineering·2021
Same author

Identifying long-term psychological distress from single measures: evidence from a nationally representative longitudinal survey of the Australian population.

BMC medical research methodology·2020

Arbitrarily primed PCR (AP-PCR) generates reproducible genomic fingerprints without prior sequence data. This method effectively distinguishes bacterial strains and plant varieties by analyzing DNA polymorphisms.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Characterizing complex genomes often requires prior sequence information.
  • Developing simple, reproducible methods for genomic analysis is crucial for various biological fields.

Purpose of the Study:

  • To introduce and validate a novel method for generating genomic fingerprints using arbitrarily primed PCR (AP-PCR).
  • To demonstrate the applicability of AP-PCR for strain differentiation across different species.

Main Methods:

  • Utilizing single, arbitrarily chosen primers and the polymerase chain reaction (PCR) for DNA amplification.
  • Employing a two-stage amplification process: initial low-stringency cycles followed by higher-stringency PCR.
  • Analyzing polymorphisms within the generated genomic fingerprints to identify differences.

Related Experiment Videos

Main Results:

  • Successfully generated simple and reproducible genomic fingerprints for complex genomes.
  • Demonstrated the ability to distinguish between different strains within species.
  • Validated the method's generality across diverse organisms, including Staphylococcus, Streptococcus pyogenes, and Oryza sativa (rice).

Conclusions:

  • Arbitrarily primed PCR (AP-PCR) is a powerful, sequence-independent technique for generating genomic fingerprints.
  • AP-PCR provides a versatile tool for strain typing and genetic diversity analysis in various species.
  • The method's simplicity and reproducibility make it broadly applicable in molecular biology and diagnostics.