[The use of a polyethyleneimine substrate for culturing dissociated cells of the central nervous system]

A new method of preparation of polyethylenimin substrate for cultivation of dissociated cells from different parts of the central nervous system is presented. The alcohol solution of polyethylenimin used for this substrate is easily prepared, needs no sterilization and can be stored unlimitedly. The substrate provides a good attachment, growth and differentiation of neurons and glial cells and possesses all the advantages of more expensive polyaminoacid substrates (polylysine, polyornithine). Our substrate is highly adhesive, displays good optical properties and no artefacts after histological and histochemical treatment.