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Estimation of allowable errors for tilt parameter determination in protein electron crystallography
B V Prasad1, L L Degn, T W Jeng
1Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030.
Ultramicroscopy
|October 1, 1990
Abstract:
A three-dimensional reconstruction of a thin protein crystal requires an accurate assignment of amplitudes and phases in the three-dimensional reciprocal space. This assignment depends upon the tilt parameters which are experimentally determined. A theoretical estimate is made of the tolerable error in the determination of tilt parameters as a function of resolution and crystal thickness.