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Updated: May 22, 2026
Processing the Loblolly Pine PtGen2 cDNA Microarray
Published on: March 20, 2009
Development of 16 microsatellite markers for Prince's Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae)
Zhenwen Liu1, Qianru Zhao1,2, Jing Zhou3
1Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Abstract:
The perennial evergreen herb, Chimaphila japonica is found exclusively in East Asian temperate coniferous or sometimes in deciduous forests. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing repeats (FIASCO) protocol, 20 microsatellite primer sets were identified in two wild populations. Of these primers, 16 displayed polymorphisms and 4 were monomorphic. The number of alleles per locus ranged from one to six among populations, values for expected and observed heterozygosities ranged from 0.000 to 0.848 and from 0.000 to 1.000, respectively. The new SSR markers will be useful in obtaining estimates of population-level genetic diversity and in phylogeographic studies of C. japonica.
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