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Related Experiment Video

Updated: May 22, 2026

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
12:04

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

A multi-platform flow device for microbial (co-) cultivation and microscopic analysis.

Matthijn C Hesselman1, Dorett I Odoni, Brendan M Ryback

  • 1Wageningen UR iGEM 2011 Team, Wageningen University, Wageningen, The Netherlands.

Plos One
|May 19, 2012
PubMed
Summary

Researchers developed a versatile flow device for microbial cultivation, enabling real-time study of gene circuits in Escherichia coli and nematode microscopy. This affordable, reusable platform supports diverse research needs.

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Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales

Published on: November 25, 2020

Area of Science:

  • Microbiology
  • Biotechnology
  • Bioengineering

Background:

  • Novel microbial cultivation platforms are crucial for studying unculturable microorganisms.
  • Advanced materials enable new high-throughput experimental designs for biological research.
  • The international Genetically Engineered Machine (iGEM) competition fosters innovation in synthetic biology tools.

Purpose of the Study:

  • To design, manufacture, and implement a versatile flow device for microbial cultivation.
  • To create a platform accommodating multiple growth substrates like microsieves and microdishes.
  • To enable controlled (co-)culturing and real-time microscopic observation of microorganisms.

Main Methods:

  • Fabrication of a flow device integrating silicon nitride microsieves and porous aluminum oxide microdishes.
  • Implementation of chemostat-like control over (co-)culturing conditions.
  • Utilization of fluorescence microscopy for real-time observation of cellular processes.

Main Results:

  • Successful demonstration of the flow device's functionality with Escherichia coli harboring synthetic gene circuits.
  • Quantitative analysis of emerging expression dynamics in real-time.
  • Demonstration of the device's utility for cultivating nematodes, indicating potential for multicellular organism studies.

Conclusions:

  • The developed flow device is affordable, reusable, and versatile for diverse microbial cultivation applications.
  • It facilitates real-time quantitative studies of gene expression dynamics in microorganisms.
  • The platform shows promise for microscopy studies of both microbial and multicellular organisms.