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Related Experiment Videos

Adipocyte lactate production remains elevated during refeeding after fasting.

F D Newby1, L K Wilson, S V Thacker

  • 1Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30303.

The American Journal of Physiology
|December 1, 1990
PubMed
Summary

Adipose tissue significantly increases lactate production after fasting. This elevated lactate production persists during refeeding, suggesting a key role for adipose tissue in replenishing liver glycogen.

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Area of Science:

  • Metabolic regulation
  • Adipose tissue biology
  • Nutrient metabolism

Background:

  • Refeeding after fasting rapidly restores hepatic glycogen.
  • Lactate is increasingly recognized as a primary substrate for glycogenesis.
  • Adipose tissue is a known site of lactate production, with fasting enhancing this activity.

Purpose of the Study:

  • To investigate the rate and duration of lactate production by isolated adipocytes from different rat adipose depots.
  • To assess changes in lactate production following a 48-hour fast and subsequent refeeding for up to 96 hours.

Main Methods:

  • Isolation of adipocytes from three distinct adipose depots in rats.
  • Measurement of glucose metabolism and lactate production in isolated adipocytes.
  • Experimental design involving a 48-hour fasting period followed by a 96-hour refeeding period.

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Main Results:

  • Fasting for 48 hours markedly increased adipocyte lactate production, raising its contribution to total glucose metabolism from 3-9% (fed) to 49-60% (fasted).
  • Elevated lactate production by adipocytes persisted for 12-24 hours into the refeeding period before declining.
  • Mesenteric adipocytes exhibited a higher rate and longer duration of elevated lactate production compared to other depots.

Conclusions:

  • Adipocyte conversion of glucose to lactate remains elevated during the critical period of hepatic glycogen restoration after fasting.
  • Adipose tissue actively contributes lactate for glycogenesis during the refeeding phase.
  • Depot-specific differences in adipocyte lactate production highlight the specialized roles of different adipose tissues in metabolic adaptation.