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Updated: May 22, 2026

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
Published on: March 13, 2013
Simplified sheath flow cuvette design for ultrasensitive laser induced fluorescence detection in capillary
Oluwatosin O Dada1, Bonnie J Huge, Norman J Dovichi
1Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA.
Abstract:
We present a design for a sheath-flow cuvette that uses a relatively inexpensive quartz cuvette. The cuvette has a high optical quality square flow chamber that is fused to quartz tubes at each end. PEEK/TEFZEL fittings hold and seal the quartz flow chamber without putting strain on the cuvette. The performance of the cuvette is evaluated as a laser-induced fluorescence detector for capillary electrophoresis. The cuvette produces mass detection limits of 50 yoctomoles (30 copies) for 5-carboxyl tetramethylrhodamine (5 TAMRA SE) with a separation efficiency of 400,000 theoretical plates.
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