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Updated: May 22, 2026
09:33
Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
Published on: February 7, 2018
Cardiovascular redox and ox stress proteomics
Vikas Kumar1, Timothy Dean Calamaras, Dagmar Haeussler
1Vascular Biology Section, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA 02118, USA.
Antioxidants & Redox Signaling
|May 22, 2012
Summary
Oxidative post-translational modifications (OPTMs) impact cardiovascular health and disease. Understanding OPTMs is key to developing new biomarkers for cardiovascular pathology and potential therapies.
Area of Science:
- Biochemistry
- Cardiovascular Biology
- Proteomics
Background:
- Oxidative post-translational modifications (OPTMs) play roles in cardiovascular physiology and pathophysiology.
- Advanced proteomic methods, including mass spectrometry (MS) and antibody identification, have increased accessibility to studying OPTMs.
- Preclinical models are clarifying the roles of enzymatically reversible OPTMs.
Purpose of the Study:
- To summarize recent advances in the identification and understanding of OPTMs.
- To highlight critical issues and limitations in current OPTM identification methodologies.
- To outline future directions for advancing OPTM research and biomarker discovery.
Main Methods:
- Mass spectrometry (MS) with advanced labeling techniques.
- Antibody-based identification methods.
- Utilizing transgenic and gene deletion animal models.
Main Results:
- OPTMs are involved in cardiovascular health and disease.
- Current identification methods for OPTMs face analytical limitations.
- Novel MS instrumentation has been crucial for discovering OPTMs and biomarkers.
Conclusions:
- OPTMs have significant potential as cardiovascular disease biomarkers and therapeutic targets.
- Standardized methods for sample processing and MS data deposition are needed.
- Further understanding of OPTM chemistry is essential for biomarker development.
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