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Updated: May 22, 2026

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing
08:04

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

Published on: May 11, 2021

Wnt-4 potently inhibits capillary outgrowth from rat aorta in 3D culture

Bernard Jover1, Daphné Girardot, Françoise de Courtois Roy de Vacquières

  • 1Groupe Rein & Hypertension, Institut Universitaire de Recherche Clinique, CNRS FRE3400, 641 Ave Doyen Giraud, 34093, Montpellier Cedex 5, France. bernard.jover@inserm.fr

Fundamental & Clinical Pharmacology
|May 22, 2012
PubMed

Insights

Wnt-4 protein inhibits new capillary growth, while Wnt-1 has no effect on this process. This study explores Wnt signaling

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Angiogenesis, the formation of new blood vessels, is a complex process regulated by cell interactions.
  • Wnt signaling pathways are implicated in the regulation of angiogenesis.

Purpose of the Study:

  • To investigate the role of Wnt-1 and Wnt-4 growth factors in capillary formation and growth.
  • To evaluate the effects of Wnt-1 and Wnt-4 on angiogenesis in vitro.

Main Methods:

  • Utilized a three-dimensional aortic ring culture system.
  • Employed ex vivo retroviral infection to introduce Wnt factors.
  • Assessed capillary outgrowth and formation in vitro.

Main Results:

  • Wnt-1 demonstrated no significant effect on capillary outgrowth.
  • Wnt-4 significantly inhibited capillary outgrowth in vitro.
  • Wnt-4 acts as a potent inhibitor of angiogenesis in this model.

Conclusions:

  • Wnt-4 is identified as a key regulator inhibiting angiogenesis.
  • The study highlights the differential roles of Wnt family members in vascular development.
  • Findings suggest Wnt-4 as a potential target for modulating angiogenesis.
Keywords:
endothelial cellsretroviral infectionthree-dimensional tissue culture

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