Expression of phosphoinositide-specific phospholipase C enzymes in normal endometrium and in endometriosis
Vincenza Rita Lo Vasco1, Martina Leopizzi, Caterina Chiappetta
1Department of Sensitive Organs, Policlinic Umberto I, Sapienza University, Rome, Italy. ritalovasco@hotmail.it
Objective:
To delineate the panel of expression of phosphoinositide-specific phospholipase C (PI-PLC) signaling enzymes in normal endometrium and in endometriosis.
Design:
Clinical/experimental study.
Setting:
University.
Patient(S):
Healthy donor woman and endometriosis-affected woman.
Intervention(S):
Normal endometrium and endometriosis surgical biopsies were analyzed using gene expression analyses methodology (reverse transcriptase-polymerase chain reaction [PCR], bioanalyses).
Main Outcome Measure(S):
Gene expression (messenger RNA concentration) measures of 12 PI-PLC enzymes: PI-PLC β1, PI-PLC β2, PI-PLC β3, PI-PLC β4, PI-PLC γ1, PI-PLC γ2, PI-PLC δ1, PI-PLC δ3, PI-PLC δ4, PI-PLC ε, PI-PLC η1, and PI-PLC η2.
Result(S):
PI-PLC β1, PI-PLC β3, PI-PLC δ1, and PI-PLC δ3 enzymes were detected, although differently expressed in normal and endometriosis tissues.
Conclusion(S):
The involvement of PI-PLC enzymes in inflammation and the consistency of susceptible endometriosis loci with PI-PLC genes mapping corroborate the hypothesis that PI signaling might be involved in the pathogenesis of endometriosis.
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