Biomass conversion determined via fluorescent cellulose decay assay
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Published on: December 12, 2013
Bente Wischmann1, Marianne Toft, Marco Malten
1Novozymes A/S, Bagsværd, Denmark.
Abstract:
An example of a rapid microtiter plate assay (fluorescence cellulose decay, FCD) that determines the conversion of cellulose in a washed biomass substrate is reported. The conversion, as verified by HPLC, is shown to correlate to the monitored FCD in the assay. The FCD assay activity correlates to the performance of multicomponent enzyme mixtures and is thus useful for the biomass industry. The development of an optimized setup of the 96-well microtiter plate is described, and is used to test a model that shortens the assay incubation time from 72 to 24h. A step-by-step procedure of the final assay is described.
