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Related Concept Videos

Chemistry of Carbohydrates03:25

Chemistry of Carbohydrates

Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
Chemistry of Carbohydrates03:25

Chemistry of Carbohydrates

Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
Chemistry of Carbohydrates03:25

Chemistry of Carbohydrates

Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
Carbohydrate Metabolism01:36

Carbohydrate Metabolism

Carbohydrates are polymers composed of molecules containing atoms of carbon, hydrogen and oxygen. One gram of carbohydrate can provide four kilo-calories of energy, which makes it the most efficient instant energy source.
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in the...

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Updated: May 22, 2026

Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases
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Published on: December 23, 2022

Quantitative approaches to the analysis of carbohydrate-binding module function.

D Wade Abbott1, Alisdair B Boraston

  • 1Lethbridge Research Station, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada.

Methods in Enzymology
|May 22, 2012
PubMed
Summary
This summary is machine-generated.

This study details four methods to analyze how carbohydrate-binding modules (CBMs) interact with polysaccharides. Understanding these CBM-polysaccharide interactions is key for enzyme function and applications.

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Last Updated: May 22, 2026

Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases
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Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
08:53

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

Published on: October 2, 2017

Area of Science:

  • Biochemistry
  • Enzymology
  • Structural Biology

Background:

  • Carbohydrate-binding modules (CBMs) are crucial for carbohydrate-active enzymes.
  • CBMs target and concentrate enzymes onto polysaccharide substrates, aiding in substrate turnover.

Purpose of the Study:

  • To present and discuss four established methodologies for investigating CBM-polysaccharide binding.
  • To explore the thermodynamics of CBM-polysaccharide interactions, including entropy-driven binding.

Main Methods:

  • Solid-state depletion assay
  • Affinity gel electrophoresis
  • UV difference and fluorescence spectroscopy
  • Isothermal titration calorimetry

Main Results:

  • The study outlines established techniques for quantifying CBM-polysaccharide binding affinities.
  • It discusses the application of these methods to understand binding thermodynamics and stoichiometry.

Conclusions:

  • Accurate quantification of CBM-polysaccharide interactions is essential for understanding enzyme mechanisms.
  • These methodologies provide a framework for characterizing CBM function in various biological and industrial contexts.