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Updated: May 22, 2026
07:10
Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae
Published on: October 8, 2013
Transformation of Clostridium thermocellum by electroporation
1Thayer School of Engineering at Dartmouth College, Hanover, New Hampshire, USA.
Methods in Enzymology
|May 22, 2012
Abstract:
In this work, we provide detailed instructions for transformation of Clostridium thermocellum by electroporation. In addition, we describe two schemes for genetic modification: allelic replacement-where the gene of interest is replaced by an antibiotic marker and markerless gene deletion-where the gene of interest is removed and the selective markers are recycled. The markerless gene deletion technique can also be used for insertion of genes onto the C. thermocellum chromosome.
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