Effect of S-TE (solubilization by thermophilic enzyme) digestion conditions on hydrogen production from waste sludge
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Updated: May 22, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
Liang Guo1, Jun Zhao, Zonglian She
1Key Laboratory of Marine Environment and Ecology, Ministry of Education, College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China. guoliang_805@yahoo.com.cn
Abstract:
S-TE (solubilization by thermophilic enzyme) digestion was always used for waste sludge solubilization and reduction with the theory of lysis enzyme secreted by thermophilic bacteria. The effects of pretreated times, pH and sludge concentrations on S-TE digestion were investigated to determine the optimum S-TE conditions on hydrogen fermentation from waste sludge. The experimental results showed that the protein, carbohydrate and SCOD (soluble chemical oxygen demand) were released after S-TE pretreatment, and the optimal conditions on sludge solubilization were 12-16 h, pH 7-9 and 4.31 g/L TSS, respectively. The optimal S-TE conditions on hydrogen yields were 8h, pH 6 and 6.83 g/L TSS (total suspended solid), and the maximum hydrogen yields were 30.80 ml/g VSS (volatile suspended solid), 33.75 ml/g VSS and 42.90 ml H2/g VSS, respectively. It was concluded that the optimal S-TE digestion conditions on sludge solubilization were different from that on hydrogen production.
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