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Initial-rate based method for estimating the maximum heterotrophic growth rate parameter (μHmax).

C Fall1, C M Hooijmans, M Esparza-Soto

  • 1Universidad Autonoma del Estado de Mexico-CIRA, Apdo postal 367, Toluca, CP 50091, Estado de Mexico, Mexico. c-fa-ll@hotmail.com

Bioresource Technology
|May 22, 2012
PubMed
Summary
This summary is machine-generated.

A new seed-increments method accurately measures heterotrophic biomass growth rate (μ(Hmax)). This approach overcomes limitations of traditional respirometry, offering more representative kinetic constants for wastewater treatment processes.

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Area of Science:

  • Environmental microbiology
  • Biochemical engineering
  • Wastewater treatment technologies

Background:

  • Respirometry is the standard for measuring maximum specific growth rate (μ(Hmax)) of heterotrophic biomass.
  • High food/microorganism ratios in batch tests may yield unrepresentative kinetic constants.
  • Existing methods lack alternatives despite recognized limitations.

Purpose of the Study:

  • To propose and validate an alternative method for measuring μ(Hmax).
  • To address the shortcomings of current respirometry-based techniques.
  • To provide a more accurate assessment of heterotrophic biomass characteristics.

Main Methods:

  • Development of seed-increments method based on initial respiration rates (r(O2)(_ini)) at varying seeding levels.
  • Utilizing ASM1-based equations to relate respiration rates to μ(Hmax).
  • Graphical analysis of r(O2)(_ini)·(V(WW)+v(ML)) versus v(ML) to determine μ(Hmax) via slope.

Main Results:

  • The seed-increments method demonstrated ease of application.
  • A consistent linear relationship was observed as predicted by the model.
  • The method successfully estimated μ(Hmax) from initial respiration rates.

Conclusions:

  • The seed-increments method offers a viable and potentially more accurate alternative to respirometry for μ(Hmax) determination.
  • This technique provides kinetic constants that better represent the biomass of interest.
  • The method is practical for application in wastewater treatment research and operations.