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Updated: May 22, 2026

Fabrication of Monolayer Graphene-Coated Grids for Cryoelectron Microscopy

Fabrication of Monolayer Graphene-Coated Grids for Cryoelectron Microscopy

Published on: September 8, 2023

Hydration-layer models for cryo-EM image simulation.

Zhiguo Shang¹, Fred J Sigworth

¹Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, 333 Cedar St., New Haven, CT 06520, USA.

Journal of Structural Biology

|May 22, 2012

Summary

This summary is machine-generated.

Related Concept Videos

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Accurate modeling of solvent density around proteins in cryo-electron microscopy (cryo-EM) is crucial. New continuum and existing binary-mask models offer rapid and precise solutions for solvated protein analysis.

Area of Science:

Structural Biology
Biophysics
Computational Biology

Background:

Quantitative comparison of cryo-electron microscopy (cryo-EM) images and 3D reconstructions with atomic structures necessitates modeling solvent electron scattering.
Molecular-dynamics (MD) simulations are the most rigorous method for determining solvating water atom density.

Purpose of the Study:

To adapt bulk-water modeling concepts from X-ray crystallography to the cryo-EM solvent-modeling challenge.
To develop and evaluate new methods for accurate solvent density modeling in cryo-EM.

Main Methods:

Adaptation of bulk-water modeling techniques for cryo-EM.
Development of a continuum solvent density model.
Comparison with the binary-mask model and MD simulations.

Related Experiment Videos

Last Updated: May 22, 2026

Fabrication of Monolayer Graphene-Coated Grids for Cryoelectron Microscopy

Fabrication of Monolayer Graphene-Coated Grids for Cryoelectron Microscopy

Published on: September 8, 2023

Main Results:

A new continuum solvent model closely matches MD-based results within sampling errors.
The established binary-mask model performs comparably to the new continuum model.
Both models provide rapid and accurate solvent density modeling.

Conclusions:

Several effective methods are now available for rapid and accurate modeling of solvated proteins in cryo-EM.
The choice of model can be guided by the required accuracy and computational resources.
These advancements facilitate more precise structural analysis from cryo-EM data.