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Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Updated: May 22, 2026

Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips
10:36

Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

Published on: February 7, 2012

A new technique for multiple re-use of planar patch clamp chips.

Liyo Kao1, Natalia Abuladze, Xuesi M Shao

  • 1Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, United States.

Journal of Neuroscience Methods
|May 22, 2012
PubMed
Summary

Researchers developed a simple protocol to reuse planar patch clamp chips, reducing experimental costs. This method enables repeated high-throughput ion channel recordings, overcoming previous limitations of single-use chips.

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Area of Science:

  • Biophysics
  • Electrophysiology
  • Cell Biology

Background:

  • The patch clamp technique is crucial for ion channel research, with planar chips offering advantages in throughput and ease of use.
  • Current planar patch clamp systems, like the Nanion Port-a-Patch, require single-use chips due to cellular debris accumulation, increasing experimental costs.
  • The ~1μm aperture in planar chips is susceptible to debris, hindering GΩ seal formation and chip reuse.

Purpose of the Study:

  • To develop and validate a simple protocol for the reuse of Nanion planar patch clamp chips.
  • To address the cost and waste associated with single-use planar patch clamp chips.
  • To demonstrate the feasibility of reusing chips for ion channel recording and drug screening.

Main Methods:

  • Developed a novel protocol for cleaning and reusing Nanion planar patch clamp chips.
  • Utilized whole-cell patch clamp recordings on HEK-293 cells expressing the NBCe1-A cotransporter.
  • Tested chip reusability with CHO-K1 cells incorporating gramicidin channels and rapid solution exchange.

Main Results:

  • Successfully demonstrated a simple protocol for the reuse of Nanion planar patch clamp chips.
  • Achieved reliable GΩ seal formation and stable recordings after chip reuse.
  • Validated the reusability across different cell types and experimental conditions, including solution changes.

Conclusions:

  • The developed protocol enables the cost-effective reuse of planar patch clamp chips, enhancing experimental throughput.
  • This breakthrough overcomes a significant limitation in planar patch clamp technology, making it more accessible and sustainable.
  • Reusable planar patch clamp chips facilitate efficient ion channel studies and drug screening applications.