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Anti-tumor activity of Annona squamosa seeds extract containing annonaceous acetogenin compounds
Yong Chen1, Sha-sha Xu, Jian-wei Chen
1College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.
Ethnopharmacological Relevance:
Seeds of Annona squamosa L. have been used in the south of China as a folk remedy to treat "malignant sores" (cancer).
Aim Of The Study:
To investigate the chemical constituents and the anti-tumor activity of the standardized A. squamosa seeds extract in vitro and in vivo.
Materials And Methods:
Annonaceous acetogenin profiles of the standardized extract were determined by using Fourier transform infrared (FT-IR) and high performance liquid chromatography (HPLC) techniques. The anti-tumor activity of the extract was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) cytotoxicity in vitro and H(22) hepatoma cells transplantation tumor model in vivo.
Results:
The FT-IR spectroscopy showed the presence of annonaceous acetogenin compounds in the extract. Two major annonaceous acetogenins: 12, 15-cis-squamostatin-A and bullatacin were identified and quantified by HPLC. The seed extract showed significant anti-tumor activity against four human tumor cell lines, especially for MCF-7 (IC(50). 0.25 μg/ml) and Hep G2 (IC(50). 0.36 μg/ml) cells in vitro. The extract inhibited the growth of H(22) tumor cells in mice with a maximum inhibitory rate of 69.55% by oral administration.
Conclusion:
A. squamosa seed extract showed significant anti-tumor activities against human hepatoma cells in vitro and in vivo, indicating a potential for developing the extract as a novel anti-liver cancer drug.
