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Updated: May 22, 2026

Quality-Controlled Sputum Analysis by Flow Cytometry
Published on: August 9, 2021
Analytical evaluation of Sysmex UF-1000i for flow cytometric analysis of peritoneal fluid
Sabrina Buoro1, Rosanna Gustinetti, Paola Dominoni
1Laboratorio Analisi Chimico Cliniche, AO Ospedali Riuniti Bergamo, Bergamo, Italy.
Objective:
To evaluate analytical performance of Sysmex UF-1000i for peritoneal fluid analysis.
Methods:
Functional sensitivity, imprecision, linearity and comparison studies were performed on peritoneal fluids.
Results:
Total imprecision was 1.6-4.7%, functional sensitivity 27/μL for white blood cell (WBC) and 32/μL for total nucleated cell (TNC) count. Linearity was excellent up to 983 cell/μL, carry-over <0.2%, correlation with manual microscopy always greater than 0.992.
Conclusion:
The instrument exhibited optimal performance at the conventional WBC diagnostic thresholds.
