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Updated: May 22, 2026
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Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
Published on: August 5, 2022
A sensitive electrochemical biosensor for detection of DNA methyltransferase activity by combining DNA
Huiwang Wu1, Shucheng Liu, Jianhui Jiang
1State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.
Chemical Communications (Cambridge, England)
|May 22, 2012
Abstract:
A novel electrochemical biosensor was developed for activity assay of DNA methyltransferase and its inhibitor based on methylation-sensitive cleavage, which activated a primer for terminal transferase-mediated extension of biotinylated dUTP followed by sensitive detection via enzymatic amplification.
