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Updated: May 22, 2026

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
Published on: August 5, 2011
Cryopreservation of shoot tips and meristems: an overview of contemporary methodologies
Erica E Benson1, Keith Harding
1Conservation, Environmental Science and Biotechnology, Damar, Cupar Muir, Fife, Scotland, UK. e.benson-damar@tiscali.co.uk
Abstract:
Cryopreservation is the storage of viable bioresources at ultra-low temperatures in liquid nitrogen (LN). This chapter provides an overview of those protocols most commonly used to cryopreserve in vitro derived shoot tips and meristems; they are described generically, as sequential technical steps, including preparative and cryogenic treatments and the morphogenetic assessment of recovery. The importance of translating research-generated methods into formal Standard Operating Procedures (SOPs) is considered.
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