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Related Concept Videos

Bacterial Transformation01:33

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Related Experiment Video

Updated: May 22, 2026

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
10:28

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Published on: February 14, 2020

Biolistic- and Agrobacterium-mediated transformation protocols for wheat.

Cecília Tamás-Nyitrai1, Huw D Jones, László Tamás

  • 1Centre for Agricultural Research, Hungarina Academy of Sciences, Martonvásár, Hungary.

Methods in Molecular Biology (Clifton, N.J.)
|May 22, 2012
PubMed
Summary

Developing genetically modified (GM) wheat is crucial for global food security. This chapter details protocols for producing transgenic wheat plants using biolistic and Agrobacterium-mediated DNA delivery for improved crop quality.

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Area of Science:

  • Agricultural Science
  • Plant Biotechnology
  • Genetics

Background:

  • Wheat is a vital global food source, with increasing demand for high-quality flour.
  • Genetically modified (GM) wheat is anticipated to be commercially available within 10-15 years, presenting new opportunities for crop improvement.

Purpose of the Study:

  • To summarize key features and challenges in wheat transformation.
  • To provide detailed protocols for producing transgenic wheat plants via biolistic and Agrobacterium-mediated DNA delivery.

Main Methods:

  • Protocols focus on explant selection (immature embryo-derived scutella) and embryogenic callus induction.
  • Regeneration of transgenic plants involves increasing selection pressure, illustrated with specific plasmids (pAHC25, pAL156) for herbicide selection.
  • Methods are applicable to both bread wheat (Triticum aestivum L.) and durum wheat (T. turgidum L. var. durum).

Main Results:

  • Detailed methodologies for obtaining stably transformed, fertile transgenic wheat plants are presented.
  • Procedures for screening and testing the resulting transgenic wheat plants are discussed.

Conclusions:

  • Successful transformation protocols are established for both bread and durum wheat varieties.
  • These methods pave the way for the commercialization of GM wheat, enhancing crop quality and yield potential.