Cloning and molecular characterization of a putative bZIP transcription factor VvbZIP23 from Vitis vinifera

The proteins harboring bZIP domains comprise a large family and play key roles in many cellular processes, one of them being tolerance to biotic and abiotic stresses in plants. In the present study, we characterize a putative bZIP transcription factor from Vitis vinifera namely VvbZIP23. Our studies revealed that a GFP fusion of VvbZIP23 is localized in the nucleus showing VvbZIP23 codes for a nuclear localized protein. VvbZIP23 identified by in silico approaches from grapevine DNA databases available in the public domain NCBI is present in a single copy in the grapevine genome as shown by Southern blot analysis. Expression of VvbZIP23 is induced by a wide spectrum of abiotic stresses, including drought, salt, and cold. Exogenous application of signaling chemicals like abscisic acid, methyl viologen, salicylic acid, jasmonic acid, and ethephon also induced expression of VvbZIP23. This shows that VvbZIP23 is involved in regulating a number of stress responses in V. vinifera. The 5' proximal region of VvbZIP23 contains many cis-acting elements, which show induction of VvbZIP23 expression in multiple stress responses. Transcripts of VvbZIP23 were found in many parts of the grapevine plant with the highest expression detected in leaves. Further in silico analysis shows that the open reading frame of VvbZIP23 is 822 bp long and codes for a 273 amino acid long protein having a characteristic bZIP domain in its N-terminal end. Overexpression of VvbZIP23-GFP fusion protein in grapevine callus leads to enhanced transcript levels of genes, homologues of which are reported to be important in regulating many stress conditions.