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In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples
Published on: August 31, 2018
Chondrosterins A-E, triquinane-type sesquiterpenoids from soft coral-associated fungus Chondrostereum sp
Hou-Jin Li1, Ying-Lu Xie1, Zhong-Liang Xie2
1School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
Abstract:
The marine fungus Chondrostereum sp. was collected from a soft coral Sarcophyton tortuosum from the South China Sea. This fungus was cultured in potato dextrose broth medium and the culture broth was extracted with EtOAc. Five new triquinane-type sesquiterpenoids, chondrosterins A-E (1-5), and the known sesquiterpenoid hirsutanol C (6), were isolated. The structures were elucidated mainly on the basis of NMR, MS, and X-ray single-crystal diffraction data. Chondrosterin A (1) showed significant cytotoxic activities against cancer lines A549, CNE2, and LoVo with IC(50) values of 2.45, 4.95, and 5.47 μM, respectively.
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