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Related Experiment Video

Updated: May 22, 2026

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

Published on: May 30, 2017

A one dose experimental cholera vaccine.

Meghan Muse¹, Cyrille Grandjean, Terri K Wade

¹Dartmouth College, Lebanon, NH, USA.

FEMS Immunology and Medical Microbiology

|May 23, 2012

Summary

This summary is machine-generated.

Developing a single-dose cholera vaccine requires understanding Vibrio cholerae (Vc) antigens. Inaba LPS is a promising antigen, but controlling its antibody dominance is key for durable immunity.

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Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages

Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages

Published on: May 30, 2013

Area of Science:

Microbiology
Immunology
Vaccine Development

Background:

Epidemic cholera control is hampered by the number of vaccine doses needed for immunity.
Individual Vibrio cholerae (Vc) colonization factors are protective antigens, but their efficacy in single-dose vaccines is unproven.
Identifying optimal Vc immunogens for durable, single-dose cholera subunit vaccines is crucial.

Purpose of the Study:

To evaluate the protective immunity induced by single doses of various Vc colonization factors.
To assess the immunogenicity and protective capacity of Vc protein and LPS antigens.
To determine the influence of Vc culture conditions on vaccine-induced protection.

Main Methods:

Immunization of subjects with single doses of Vc colonization factors (Inaba LPS, TcpA, TcpF, CBP-A) emulsified in adjuvant.
Measurement of vibriocidal and antibody titers (IgG) post-immunization.
Assessment of protective efficacy against Vc challenge under different culture conditions (AKI vs. standard).

Main Results:

Inaba LPS consistently induced vibriocidal and protective antibodies, with a booster enhancing protection.
Vc protein immunogens showed variable immunogenicity; CBP-A induced higher IgG titers than TcpA or TcpF.
Protection from TcpA or TcpF antisera was dependent on Vc challenge culture conditions; CBP-A lacked consistent protection.

Conclusions:

Leveraging the right Vc immunogens is essential for achieving durable immunity with single-dose cholera subunit vaccines.
The dominant anti-LPS antibody response needs to be modulated to uncover additional protective antigens.
Further research is required to develop effective single-dose cholera vaccines combining multiple Vc antigens.