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Updated: May 22, 2026
Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
Published on: April 21, 2023
Antifungal and physical characteristics of modified denture base acrylic incorporated with silver nanoparticles
Ki-Young Nam1, Cheong-Hee Lee, Chul-Jae Lee
1Department of Dentistry, College of Medicine, Keimyung University, Dongsan-dong, Jung-gu, Daegu, Korea. nkyp@dsmc.or.kr
Objective:
This study evaluated the antifungal and physical characteristics of denture base acrylic combined with silver nanoparticles.
Materials And Methods:
Polymerized denture acrylic disc specimens containing 0 (control), 1.0, 5.0, 10.0, 20.0 and 30.0 wt% of silver nanoparticles were placed on separate culture plate dish and 100 ìL samples of yeast suspension of Candida albicans strain were inoculated on each specimens and incubated at 37°C, for 24 h. The antifungal effects were evaluated as a number of viable cells in retrieved fungal suspension. To characterize physical aspects, specimens were tested for elution of silver cation (Ag(+)) at 24 h and 30th day, thermal analysis (TG/DTA), scanning electron microscope and energy dispersed X-ray analysis (SEM/EDX) and color stability.
Results:
Significant reduced CFU was exhibited at 20.0 and 30.0 wt% of silver nanoparticles incorporated (p < 0.01) and Ag(+) elution from specimens (maximum 0.356 ± 0.11 mg/L) contributed little to the antifungal activity considering MIC of Ag(+) in this study (3.0 mg/L). The successful synthesis of modified denture acrylic containing silver nanoparticles was accessed by TG/DTA and EDX analysis.
Conclusion:
The modified denture base acrylic combined with silver nanoparticles displayed antifungal properties and acted like latent antifungal material itself with low-releasing Ag(+), however, the improvement of poor color stability was still required.
