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Updated: May 22, 2026
The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues
Published on: June 16, 2018
[Establishment and characterization of a human lung adenocarcinoma cell line, Ch-Huang-1]
Bailing Li1, Keng Zhong, Lei Jin
1Department of Cardiothoracic Surgery, Changhai Hospital, the Second Military Medical University, Shanghai 200433, China.
Background And Objective:
Lung cancer is a major worldwide health problem. The aim of this study is to establish a novel Chinese human lung adenocarcinoma cell line and examine its biological characteristics.
Methods:
Lung adenocarcinoma specimens were freshly resected during surgery. The tissues were incubated in vitro and the cell line was named Ch-Huang-1. The biological characteristics of the cells were investigated by light microscopy, chromosome analysis, and transplantation experiment.
Results:
Light microscopy revealed that cells from the primary tumor, Ch-Huang-1 cell line, and transplanted tumor possessed the characteristics of a malignant glandular epithelial tumor. The cell growth curve, doubling time, and mitotic index were also observed in vitro. Nuclear chromosome analysis revealed that the tumor was a subtriploid with a mode of 35-44 per cell. Tumor nodes were observed under the skin of nude mice by heterogenic transplantation.
Conclusion:
The characteristics of the established cell line suggest that it is a newly established human adenocarcinoma cell line.
