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Updated: May 22, 2026

Inducing Apical Periodontitis in Mice

Inducing Apical Periodontitis in Mice

Published on: August 6, 2019

Microbial diversity in failed endodontic root-filled teeth.

Chen Zhang¹, Ben-xiang Hou, Huan-ying Zhao

¹School of Stomatology, Capital Medical University, Beijing 100050, China.

Chinese Medical Journal

|May 23, 2012

Summary

This summary is machine-generated.

Persistent root canal infections cause endodontic treatment failure. 16S rRNA sequencing identified diverse bacteria, including novel pathogens, in failed root canals, highlighting inter-sample variability and potential new targets for treatment.

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Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

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Published on: October 20, 2011

Area of Science:

Microbiology
Endodontics
Molecular Biology

Background:

Persistent intraradicular infections are a primary cause of endodontic treatment failure.
Understanding the microbial communities in these infections is crucial for improving treatment outcomes.

Purpose of the Study:

To investigate the bacterial diversity in root-filled teeth with persistent intraradicular infections using 16S rRNA sequencing.
To identify candidate pathogens contributing to endodontic treatment failure.

Main Methods:

DNA extraction from 15 infected root canal samples.
Polymerase chain reaction (PCR) amplification of the 16S rRNA gene.
Cloning and sequencing of PCR amplicons to identify bacterial phylotypes.

Main Results:

All samples showed positive PCR amplification, confirming bacterial presence.
Sixty-five phylotypes from seven phyla were identified, with an average of 9.4 phylotypes per sample.
Prevalent species included *Parvimonas micra*, *Solobacterium moore*, *Dialister invisus*, *Enterococcus faecalis*, *Filifactor alocis*, and *Fusobacterium nucleatum*. Nineteen uncultivated phylotypes were also detected.

Conclusions:

Persistent intraradicular infections are consistently present in failed endodontic treatments.
The study identified novel candidate endodontic pathogens, including uncultivated bacteria, involved in post-treatment apical periodontitis.