Impact of normalization method on experimental outcome using RT-qPCR in Staphylococcus aureus

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Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
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Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Published on: November 4, 2016

Lukas Valihrach1, Katerina Demnerova

  • 1Institute of Chemical Technology, Prague, Faculty of Food and Biochemical Technology, Department of Biochemistry and Microbiology, Prague, Czech Republic. valihral@vscht.cz

Journal of Microbiological Methods
|May 23, 2012
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...