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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
Published on: June 12, 2018
What comes first: translational repression or mRNA degradation? The deepening mystery of microRNA function
1Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
Cell Research
|May 23, 2012
Abstract:
While many mechanisms have been proposed for microRNAs (miRNAs) function, most ultimately cause message degradation. A view has emerged that miRNAs silence gene expression by promoting the association of mRNA decay factors. Recent research results, however, suggest that in both zebrafish and fruit fly, translational inhibition is the initiating event of miRNA-mediated gene silencing.
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