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Retinoblastoma protein-interacting zinc-finger gene 1 (RIZ1) dysregulation in human malignant meningiomas

Z Y Liu1, J Y Wang, H H Liu

  • 1Department of Neurosurgery, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China.

Oncogene
|May 23, 2012
PubMed

Insights

Retinoblastoma protein-interacting zinc-finger gene 1 (RIZ1) is downregulated in malignant meningiomas. Restoring RIZ1 expression inhibits tumor cell growth, suggesting RIZ1 is a potential tumor suppressor for meningiomas.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Retinoblastoma protein-interacting zinc-finger gene 1 (RIZ1) is frequently silenced in human cancers.
  • The role of RIZ1 in meningioma progression, particularly malignant meningiomas, is not well understood.

Purpose of the Study:

  • To investigate the association between RIZ1 gene expression and the progression of meningiomas.
  • To determine the functional role of RIZ1 in malignant meningioma cells.

Main Methods:

  • Analysis of Affymetrix GeneChip microarray data.
  • Validation using quantitative PCR (qPCR), western blot, and immunohistochemistry.
  • In vitro functional assays including proliferation, cell cycle, invasion, and apoptosis assays after RIZ1 overexpression via lentivirus transfection.

Main Results:

  • RIZ1 expression is significantly downregulated in malignant meningiomas compared to benign ones.
  • Forced RIZ1 expression in malignant meningioma cells inhibited proliferation and induced G2/M cell cycle arrest.
  • RIZ1 overexpression promoted apoptosis and was associated with decreased c-myc expression.

Conclusions:

  • RIZ1 expression decreases with meningioma progression.
  • RIZ1 functions as a tumor suppressor in malignant meningiomas.
  • RIZ1 may be a potential therapeutic target for meningiomas.

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