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Related Experiment Video

Updated: May 22, 2026

Design and Development of Aptamer&#8211;Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
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Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

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An aptamer-based fluorescence biosensor for multiplex detection using unmodified gold nanoparticles.

Fang Luo1, Lingyan Zheng, Shanshan Chen

  • 1Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, Fujian Province Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, 350002, China. zylin@fzu.edu.cn gnchen@fzu.edu.cn.

Chemical Communications (Cambridge, England)
|May 23, 2012
PubMed
Summary
This summary is machine-generated.

A new aptamer-based biosensor enables multiplex detection of adenosine, thrombin, and cocaine using unmodified gold nanoparticles (AuNPs). This versatile fluorescence method can also detect other small molecules.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Multiplex detection is crucial for diagnosing complex diseases and monitoring multiple analytes simultaneously.
  • Existing biosensors often require complex modifications or expensive reagents, limiting their practical application.
  • Aptamer-based biosensors offer high specificity and affinity for target molecules.

Purpose of the Study:

  • To develop a novel aptamer-based fluorescence biosensor for the simultaneous detection of adenosine, thrombin, and cocaine.
  • To utilize unmodified gold nanoparticles (AuNPs) for simplified sensor construction.
  • To demonstrate the versatility of the developed strategy for detecting other small molecule substances.

Main Methods:

  • Fabrication of a fluorescence biosensor using unmodified gold nanoparticles (AuNPs).
  • Design and selection of specific aptamers for adenosine, thrombin, and cocaine.
  • Optimization of fluorescence signal detection for multiplex analysis.

Main Results:

  • The developed biosensor successfully achieved multiplex detection of adenosine, thrombin, and cocaine.
  • The sensor demonstrated high sensitivity and specificity for the target analytes.
  • The strategy proved adaptable for detecting other small molecule targets, showcasing its broad applicability.

Conclusions:

  • A novel, efficient, and versatile aptamer-based fluorescence biosensor has been developed.
  • The use of unmodified AuNPs simplifies the biosensor fabrication process.
  • This platform holds significant potential for various diagnostic and analytical applications.