Related Experiment Video
Updated: May 22, 2026
12:01
A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
Microsatellite markers for Bistorta vivipara (Polygonaceae)
Unni Vik1, Tor Carlsen, Pernille Bronken Eidesen
1Microbial Evolution Research Group, Department of Biology, University of Oslo, P.O. Box 1066 Blindern, NO-0316 Oslo, Norway. unni.vik@bio.uio.no
American Journal of Botany
|May 23, 2012
Summary
Researchers developed new microsatellite markers for Bistorta vivipara, a plant species. These genetic markers effectively distinguish between individual plants and their clones, aiding in population studies.
Area of Science:
- Genetics
- Botany
- Ecology
Background:
- Bistorta vivipara is an arctic-alpine perennial herb.
- Distinguishing between genets (individual genetic individuals) and ramets (clonal offshoots) is crucial for population studies.
Purpose of the Study:
- To develop and characterize microsatellite markers for Bistorta vivipara.
- To enable differentiation between genets and ramets within populations of B. vivipara.
Main Methods:
- Genomic shotgun 454 sequencing was employed to identify candidate microsatellite markers.
- Polymorphism screening was conducted on seven test samples.
- A multiplex protocol was developed for selected markers.
Main Results:
- Out of 50 candidate microsatellite markers, 31 were found to be polymorphic.
- A multiplex protocol was successfully developed for 16 of these polymorphic markers.
Conclusions:
- The developed microsatellite markers are effective tools for studying genetic variation in Bistorta vivipara.
- These markers facilitate the distinction between genets and ramets, enhancing population genetic research.
