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Updated: May 22, 2026

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Microsatellite primers for the endangered aquatic herb, Ottelia acuminata (Hydrocharitaceae)
Chao Xu1, Zhi-Yuan Du, Jin-Ming Chen
1Laboratory of Plant Systematics and Evolutionary Biology, College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, People's Republic of China.
Premise Of The Study:
Microsatellite primers were developed in the endangered aquatic herb, Ottelia acuminata, to characterize its genetic diversity and understand its population structure.
Methods And Results:
Eight polymorphic microsatellite markers were developed from two populations of O. acuminata in China. The number of alleles per locus ranged from one to 15; the observed and expected heterozygosities ranged from 0 to 0.885 and from 0 to 0.888, respectively, in the two populations. Selected loci also amplified successfully in O. sinensis.
Conclusions:
These microsatellite markers will facilitate further studies on the conservation genetics and evolutionary history of O. acuminata.

