Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Hyperosmolality activates polycystin-2 and TRPM4 in renal primary cilium.

Pflugers Archiv : European journal of physiology·2024

Same author

Regenerative Calcium Currents in Renal Primary Cilia.

Frontiers in physiology·2022

Same author

Inward Ca<sup>2+</sup> current through the polycystin-2-dependent channels of renal primary cilia.

American journal of physiology. Renal physiology·2021

Same author

The TRPP2-dependent channel of renal primary cilia also requires TRPM3.

PloS one·2019

Same author

Steady-state centrifugal input via the lateral olfactory tract modulates spontaneous activity in the rat main olfactory bulb.

Neuroscience·2017

Same author

Primary cilia regulate the osmotic stress response of renal epithelial cells through TRPM3.

American journal of physiology. Renal physiology·2017

Search research articles

Related Experiment Video

Updated: May 22, 2026

In Vivo Electrophysiological Measurement of Compound Muscle Action Potential from the Forelimbs in Mouse Models of Motor Neuron Degeneration

In Vivo Electrophysiological Measurement of Compound Muscle Action Potential from the Forelimbs in Mouse Models of Motor Neuron Degeneration

Published on: June 15, 2018

A selective PMCA inhibitor does not prolong the electroolfactogram in mouse.

Edwin R Griff¹, Nancy K Kleene, Steven J Kleene

¹Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio, United States of America.

|May 23, 2012

Summary

Calcium removal is crucial for ending odor responses in olfactory neurons. This study found that while sodium-calcium exchange is important, plasma membrane calcium-ATPase (PMCA) does not significantly contribute to this calcium clearance.

More Related Videos

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

Published on: May 22, 2019

Long-term Potentiation of Perforant Pathway-dentate Gyrus Synapse in Freely Behaving Mice

Long-term Potentiation of Perforant Pathway-dentate Gyrus Synapse in Freely Behaving Mice

Published on: November 29, 2013

Related Experiment Videos

Last Updated: May 22, 2026

In Vivo Electrophysiological Measurement of Compound Muscle Action Potential from the Forelimbs in Mouse Models of Motor Neuron Degeneration

In Vivo Electrophysiological Measurement of Compound Muscle Action Potential from the Forelimbs in Mouse Models of Motor Neuron Degeneration

Published on: June 15, 2018

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

Published on: May 22, 2019

Long-term Potentiation of Perforant Pathway-dentate Gyrus Synapse in Freely Behaving Mice

Long-term Potentiation of Perforant Pathway-dentate Gyrus Synapse in Freely Behaving Mice

Published on: November 29, 2013

Area of Science:

Neuroscience
Cell Biology
Sensory Physiology

Background:

Calcium ions (Ca2+) accumulate in olfactory receptor neuron cilia during odor transduction.
Efficient Ca2+ removal is essential for terminating olfactory responses.
Both Na+/Ca2+ exchange and PMCA were previously suggested to mediate Ca2+ removal.

Purpose of the Study:

To investigate the specific contribution of PMCA to Ca2+ removal during olfactory response termination.
To differentiate the roles of Na+/Ca2+ exchange and PMCA in clearing intracellular Ca2+.

Main Methods:

Measurements of odor-induced field potential termination time course in intact mouse olfactory epithelium.
Experimental manipulation using Li+ to inhibit Na+/Ca2+ exchange.
Application of a specific PMCA inhibitor, caloxin 1b1.

Main Results:

Inhibition of Na+/Ca2+ exchange by replacing Na+ with Li+ significantly prolonged response termination, as anticipated.
Treatment with the PMCA inhibitor caloxin 1b1 did not result in a significant alteration of the response termination time course.
These findings indicate that PMCA activity is not a major factor in rapid Ca2+ clearance.

Conclusions:

Under the tested experimental conditions, PMCA does not play a detectable role in the termination of olfactory responses.
Na+/Ca2+ exchange appears to be the primary mechanism for Ca2+ extrusion in this context.
Further research may be needed to fully elucidate the complex mechanisms of calcium homeostasis in olfactory neurons.