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Updated: May 22, 2026
Mouse Adipose Tissue Collection and Processing for RNA Analysis
Published on: January 31, 2018
Gender-dependent protein expression in white adipose tissues of lean and obese rats fed a high fat diet
Rajib Mukherjee1, Jung-Won Choi, Duk Kwon Choi
1Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk, Korea.
Background:
Proper understanding of molecular mechanisms underlying gender dimorphism in obesity for better nutritional recommendation is still in early stages. As white adipose tissues (WAT) is most important tissue in obesity metabolism, comparative proteomic analysis of all three WAT deposits at the same time to yield immensely important protein markers was the primary goal of this study.
Methods:
We performed differential expression analysis of protein profiles of three different WAT viz. subcutaneous, inguinal, and abdominal fat deposits of both genders in lean and obese rats fed a high fat diet (HFD) using a combination of 2-DE and MALDI-TOF-MS.
Results:
The proteomics analysis enabled us to detect 25, 29, and 46 proteins showing gender differences in three WAT deposits, respectively, to gain insight into cause of higher body weight gain in male in response to HFD.
Conclusion:
The gender dimorphism found in this proteomic study implies that female rats have a lower tendency to undergo metabolic syndrome manifestation, which is associated with lower reliance on lipid as an energy fuel, lower lipogenesis, as well as increased mitochondrial oxidative capacity. In conclusion, most of the candidate proteins identified herein by differential proteomics were previously unrecognized in gender dimorphism of adipose tissue.
