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Updated: May 22, 2026
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Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
Published on: June 16, 2015
Rolling the human amnion to engineer laminated vascular tissues.
Salma Amensag1, Peter S McFetridge
1J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
Tissue Engineering. Part C, Methods
|May 24, 2012
Summary
Researchers developed a novel method for blood vessel regeneration using decellularized human amniotic membrane. This approach creates functional vascular grafts, addressing limitations in current cardiovascular surgery options.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Vascular Biology
Background:
- Cardiovascular disease necessitates vascular grafts, but autologous vessels are limited.
- Developing functional, biodegradable vascular scaffolds remains a significant challenge.
Purpose of the Study:
- To investigate a multilaminate rolling approach using human amnion for blood vessel regeneration.
- To assess the biocompatibility and remodeling potential of human amniotic membrane-derived vascular constructs.
Main Methods:
- Human amniotic membrane was decellularized using sodium dodecyl sulfate.
- Endothelial cells (EC) and smooth muscle cells (SMC) were cultured on the membrane.
- Constructs were rolled into multilayered conduits and cultured for 40 days.
Main Results:
- Positive EC adhesion and progressive SMC repopulation were observed.
- SMC gene expression and mechanical properties changed, indicating vessel remodeling.
- The method rapidly produced cell-dense constructs with an extracellular matrix similar to natural vessels.
Conclusions:
- The multilaminate rolling of decellularized human amnion offers a promising strategy for vascular tissue engineering.
- This technique allows for control over construct physical parameters, enabling tailored vessel specifications.
- The approach presents new avenues for regenerative applications with broad clinical impact.
