Timetables of cytogenesis in the rat subfornical organ

Timetables of neurogenesis and ependymal cell production in the rat subfornical organ (SFO) were determined by examining the offspring of pregnant rats injected with [3H]thymidine on E13-E14, E14-E156, ... E21-E22, respectively. The proportion of postmitotic cells originating each embryonic day was determined by analyzing, in the adult offspring, the progressive reduction in the proportion of labeled precursors from the maximum amount seen in the E13-E14 group. Neurogenesis was found to occur over an extended period of time, beginning on E12 and continuing through E21. Ependymal cells were generated E15 through E21. Both neuron and ependymal cell production occurred in a triphasic pattern and followed an anterior (older) to posterior (younger) gradient. The anterior to posterior production gradient may be related to the morphological variation which exists along this plane. A production gradient intrinsic to a particular levels was found only in the posterior SFO, where peripheral neurons form earlier than core neurons. That neurogenetic gradient may be related to the core-periphery topographical patterns found in other studies, and suggests that the core neurons, since they are among the last to be formed, may be interneurons.